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This view shows 5 actin subunits in the filament model arranged with the axis vertical. [Close]

Coordinate system is displayed. The helical axis has been arranged to lie parallel to Z. [Close]

Each subunit is displayed with all atoms at their Van der Waals radii. White and yellow subunits are at the "barbed end"; green and magenta are at the "pointed-end". Yellow and magenta are in the second strand. [Close]

Model for the rigor complex

The ATP free structure ( Rayment et al, 1993) is shown fitted to the actin model. The 25kD "subdomain" is in green; 50kD in red; 20kD in blue; essential light chain in yellow; regulatory light chain in magenta. [Close]

ADP complex

This is a model of ADP:actin fitted in the reconstruction of Jontes & Milligan. The lever moves 3o-35 Angstroem towards the pointed end. In muscle, there is relative translation of the actin filaments relative to myosin, so the movement would translate the filament from barbed end (Z-disk) to the pointed-end (Centre). [Close]

Show model for MDE.ADP.ALF4 complex

The rigor complex lever arm has been rotated to superimpose on the smooth muscle structure. The latter is missing the regulatory light chain and associated part of the heavy chain. So the lever arm position is modelled here, assuming that the lever moves as a rigid body. [Close]

Show model for Scallop complex - "ATP" form

The rigor complex lever arm has been rotated to superimpose on the scallop ADP structure ( Houdusse et all. Cell 97, 459-470 199). Since crystals with AMP.PNP or ADP.Be.Fx have similar cell dimensions, it is argued that this structure is a better analogue for ATP form before it binds to actin, or is weakily bound to actin, before hydrolysis of nucleotide. [Close]

Toggle between nucleotide free and transition state complex

Successive clicking of the button will toggle between nucleotide free and transition state complex models. [Close]

Spin y

Spin molecule through 360 degrees about vertical axis [Close]

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