MCB 3M
ISOLATION
& PRELIMINARY CHARACTERISATION OF (
CLD’s )
FROM MAMMALIAN
BRAIN
MONDAY CLASS
TOTALS
Ice buckets
x 9
Crude homogenate
Sucrose gradient
Eppendorf tubes
x 9 Bags
of 50
Bag of
eppendorf tubes
10 Blue tips
( End cut
off )
9 x 100
ml Distilled water
9 x
30µL 10x IVK
Buffer + Vanadate
on ice
9 x
30µL 10x IVK
Buffer - Vanadate
on ice
x2
Protein loading buffer
9 x
Whirlimixers
36 x Resealable
bags 140 x 100
mm
9
x Eppendorf racks
9
x 1 ml Finnpipetters
9
x 5 – 40 µL Finnpipetters
9
x Full boxes
of blue tips
9
x Timers
9
x Marker pens
Latex gloves
L , M ,S
Vinyl gloves
L, M, S
9
x 100µL 100Mm
ATP
Make Gels on
Monday
CC TV
video set up
8
x Autoclave bags
Microfuges x 3