MCB3 (SUMMER TERM)

ISOLATION AND PRELIMINARY CHARACTERISATION

OF (CLD,s) FROM MAMMALIAN BRAIN

 

MONDAYS (PER GROUP)                     3 p.m..   -     5 p.m.

 

Ice bucket (large)

 

Crude homogenate (on ice)  50mL  each.

 

Sucrose gradient (on ice)

 

 Eppendorf tubes 1.5ml  ( at least 50  ) in bag

 

Blue tip (end cut off)  x  1

 

100mls dist. Water

 

30ul   10X IVK buffer + vanadate (on ice)

 

30ul  10X IVK buffer – vanadate (on ice)

 

100ul  100mM ATP (on ice)

 

500ul   2x protein loading buffer (on ice)

 

Whirlimixer

 

4 Resealable plastic bags  (  140 mm x 100 mm )

 

Eppendorf rack  x  3

 

1ml Finnpipette

 

5-40ul Finnpipette

 

Yellow tips (full box)

 

Blue tips

 

Timer

 

Marker pen

 

PER CLASS

 

Latex  gloves  L,M,S                   CC  TV  VIDEO  SET UP +  video tape

 

Vinyl  gloves  L,M,S                    Microfuges  x  4