MCB3 (SUMMER TERM)

ISOLATION AND PRELIMINARYCHARACTERISATION

OF (CLD’s) FROM MAMMALIAN BRAIN

Recipe cards in red box

 

TUESDAYS (PER GROUP)                             2 p.m.  -   5 p.m

 

Set 1+2 and alternate samples from day1

 

2 SDS PAGE gels + tanks

 

1L  Tank buffer

 

150mls Transfer buffer

 

15mls Cholesterol assay reagent   (safe disposal necessary due to Na azide)

 

15ml Cholesterol calibrator   (on ice)

 

12ml Molecular weight marker  (on ice)

 

Large ice bucket

 

6mls Bio-rad Bradford concentrate

 

400ml 1mg/ml BSA (on ice)

 

Box eppendorfs ~50

 

Small  fine  spatula

 

2 Small plastic trays (eppendorf box separated)

 

2 pieces PVDF membrane pre-soaked (see recipe card)

 

8 pieces filter paper same size as PVDF membrane

 

2 envelopes

 

Distilled  water

 

Marker pen

 

Forceps

 

25ml beaker

 

10ml Finnpipette + tips

 

1ml Finnpipette

 

5-40ml Finnpipette

 

Blue tips

 

Gel  loading  tips   x  10                                 ( Fisherbrand   PQP-030G   1 - 200mL )

 

Yellow tips (full box)

 

Scalpel

 

Eppendorf  rack

 

Eppendorf rack  x  3  ( for water bath )

 

Whirlimixer

 

 

 

 

PER WORK BAY

 

Power packs for running gels + blotters

 

Spectrophotometer        500nm  then  595nm

 

PER CLASS

 

3-4 semi-dry blotters           P. Mclaughlin  Swan 3.1

                                             A. Bird            Swan 4.1

                                            D.Tollervey      Swan  5.1

 

 Cuvettes                    BDH  semi micro  0.5 – 2ml  307/3800/04

 

Latex gloves

 

Vinyl  gloves

 

95 ⁰ C   Water bath  or  Heating  block  x  4

 

37⁰  C   Water  bath  x  8

 

CCTV  SET  UP