Overview

• Students watch video
• Remind them that although caveolae contain only 2% total cell protein, contain 98% PrP protein ( and src, kinases, siganlling complexes).
• Invite them to sort themselves into 8 groups of 5

Announcements

  Remind them to use wide-bore tip to remove fractions

  To save time and materials we only take every third fraction.

  Explain Vanadate

  Remind them to label bags so that can find them - separate Vanadate plus/minus

  Remind to keep a crude and pellet sample

  Use gloves in preparing kinase assays

Safety

Fix up those without a lab' coat.  If so, warn that you are doing them a favour and could send them out of lab'.

Equipment

          Nothing specific.