Overview

• Very hard day in which bulk of work of whole practical done.
• They run gels and blot them onto PVDF. They do both cholesterol and protein assays.
• As soon as they come in the door, get them to heat their gel samples. Don't slow them up by giving a talk. It is critical that the gels are on as soon as possible.
  
They can save time if two people working together load one gel on each side simultaneously

• Summary of power pack settings. Default of 40mA when running two gels, but can run at 80mA for two if gel is slow. For semi-dry blots, 100mA for each stack. 10V should do this for six stacks. NEVER go above a total of 25V.
  

Announcements

  Must be very organised. Get gels on early. Do protein and cholesterol assays while gels running.

  Read protein and cholesterol assays all way through before starting. If you don't you will find that you have missed something and this will double the time you need spend in lab'.

Keep tank buffer for next day.

Gels are already, set up to save time.

When disassembling the gel, run a spatula along one edge and twist gently until the seal breaks. Pressure applied to the edge only will most likely break the plates.

Wet your (gloved) fingers when removing the gel from the plates: it is less likely to break.

Safety

• Announce that they should not put on the current to gels until apparatus is completely assembled ( and should switch off at mains before they disassemble it)

 

Equipment

         Gel tanks
         Power Packs
         Blotting
         Spectrophotometer

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