MCB Practical 2
Isolation and Characterization of Caveolar Lipid Domains
Equipment - Gel apparatus
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Gel apparatus will take two gels. (If only one gel, use spare back-plate
to seal the other position)
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Fill up the assembled apparatus to the "Max'" mark.
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Run the voltage at 20mA per gel. As the gel runs, increase voltage to maintain
current. If in a hurry, you can run at limiting 150V. Demonstrators 2001 say that ran at 40mA each gel. Needed to do this to get out by 6pm. No adverse effects on the gels or apparatus were reported.
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There any many different sorts of power packs. The worst is the newest
( GIBRL 2000mA). The mA must be set to 60 before it will produce a load.
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Dismantle carefully.Insert a spatula along an edge ( not notched!) and
rotate it slowly until the seal between the plates breaks. Wet fingers
of gloved hand before removing the gel.