Protein detection by Western-blot is often performed by revealing peroxidase activity of secondary antibodies.

For that one uses ECL or other equivalent reagent, which are quite expensive.

Here is so the recipe of a "home-made ECL":

1) Prepare the following stock solutions:

2) Prepare the following solutions:

Store these two solutions in fridge, wrapped in aluminium foil for about 2 months, after which sensitivity may decay.

Reveal your western-blots with a 1:1 mix of solution 1 and 2 as you would do with commercial ECL reagent.